5th International Conference on Cancer Genomics
Las Vegas, USA
Young Hee Joung
Chonnam National University, South Korea
Title: Development of colorectal carcinoma vaccine candidates using plant expression GA733-Fc
Biography
Biography: Young Hee Joung
Abstract
The GA733-2, colorectal carcinoma associated antigen and epithelial cell adhesion molecule (EpCAM), is highly expressed on the surfaces of human colorectal carcinoma cells. It was utilized to produce a vaccine for colorectal cancer in a plant system. To develop a vaccine using a plant system, recombinant protein rGA733-2 and rGA733-2-Fc genes were cloned into plant expression vector (pBINPLUS) with an ER targeted signal peptide and KDEL retention codon. The cloned vectors were introduced into tobacco plants using Agrobacterium mediated stable transformation or agro-infiltrated transient transformation. The recombinant proteins, rGA733-2, rGA733-Fc and Fc were purified from transgenic tobacco leaves and evaluate their anti-cancer effects using MC38 cancer cells. Only the rGA733-Fc showed anti-cancer effects. To develop an edible colorectal cancer vaccine, the rGA733-Fc gene was introduced into tomato plants (Solanum lycopersicum cv. Micro-Tom). The rGA733- Fc expressed transgenic tomato fruits were administered orally to C57BL/6J mic and then colorectal cancer was induced in the oral treatment mice using MC38 cells injection. The plant derived rGA733-Fc treated mice showed amelioration of colorectal cancer is caused by rGA733-Fc induced anti-cancer immune response stimulation.