5th International Conference on Cancer Genomics
Las Vegas, USA
Yang Do Choi
Seoul National University, South Korea
Title: Modulation of growth and stress response through genome editing by CRYSPR/CAS9 system
Biography
Biography: Yang Do Choi
Abstract
Jasmonic acid (JA) is an important hormone mediating stress response in plant. JA response has been widely studied for the purpose of developing stress-resistant transgenic crops. Increasing numbers of studies reported that enhanced JA response improves resistance against stresses, but also induces negative effects on plant growth and productivity. Understanding of JA inhibition mechanism against growth is essential to develop transgenic plants carrying both stress-resistance and enhanced growth. To understand the JA-mediated growth inhibition mechanism, function of OsJAZ9 responsible for JA signaling was studied through genome editing by CRYSPR/CAS9 system. We traced temporal and spatial expression pattern of OsJAZ9, and also analyzed its mutant Osjaz9 generated by CRISPR/Cas9 system that can modify response of target genes with high accuracy. Through Agrobacterium-mediated callus transformation and selection, we collected 30 individual transgenic plants. Among them 8 individual plants (around 30%) carried mutated OsJAZ9 gene in their genome. From this study we found that expression pattern of OsJAZ9 changes along developmental stages, predominant expression in leaf at early developmental stage but in roots at late developmental stage. Further characterization of Osjaz9 mutants will expand our understanding how JA allows plants to coordinate dynamics of stress response and growth.