5th International Conference on Cancer Genomics
Las Vegas, USA
Rajesh R Singh
University of Texas MD Anderson Cancer Center, USA
Title: Size matters: Challenges in implementing large Next-generation sequencing panels for routine clinical screening of tumors
Biography
Biography: Rajesh R Singh
Abstract
Massively parallel sequencing capability of Next-generation sequencing technologies have made them genome sequencing platforms of choice for routine screening of clinically significant markers in tumors. Most popular approach generally includes screening of limited areas (mutational hotspots) of limited number of relevant genes in order to restrict costs and practical turn-around-time (TAT) for the results. However, rapid discovery of markers warrants increasing the number genes for routine screening which is challenging due to increased costs, complexity of interpretation and decreasing sequencing throughput. In this talk, the experience of implementing progressively large gene panels in a high-volume molecular diagnostic laboratory will be highlighted. The logistic issues of implementing large NGS panels and the steps taken to meet these challenges in the laboratory will be discussed.